Helen Mackie received a B.Sc.. Honours at Queen's University in Biology and Chemistry in 1943, and in 1949 she received a M.Sc. in Physiology and Biochemistry from the University of Toronto. In 1973, Mackie received a B.F.A. from the University of Calgary in Printmaking and Drawing.
She has participated in numerous group exhibitions, including the Taejon Expo International Exhibition of Graphic Arts, Taejon, Korea (1993), the Alberta Society of Artists 65th Anniversary Exhibition (1996), the 5th International Biennal Print Exhibit: 1991 ROC, Taipei, Taiwan (1991), 1st Kochi International Triennial Exhibition of Prints, Kochi-shi, Japan (1990), and International Exhibition Small Forms of Graphic Art, Lodz, Poland (1985-1989). She has had many solo exhibitions as well; she exhibited at Giordano Gallery, Edmonton (1993), Whyte Museum of the Canadian Rockies, Banff (1987), and Kanagawa International Association, Kanagawa, Japan (1984), amongst many others.
Mackie's work can be found in the collections of City of Calgary Civic Collection, Glenbow Museum, H.R.H. Queen Elizabeth II Permanent Collection, Windsor Castle Library, England, the Alberta Foundation for the Arts, as well as others.